Journal article
Mouse guanylate-binding protein 1 does not mediate antiviral activity against influenza virus in vitro or in vivo
MB Tessema, DE Tuipulotu, CV Oates, AG Brooks, SM Man, SL Londrigan, PC Reading
Immunology and Cell Biology | Published : 2023
DOI: 10.1111/imcb.12627
Abstract
Many interferon (IFN)-stimulated genes are upregulated within host cells following infection with influenza and other viruses. While the antiviral activity of some IFN-stimulated genes, such as the IFN-inducible GTPase myxoma resistance (Mx)1 protein 1, has been well defined, less is known regarding the antiviral activities of related IFN-inducible GTPases of the guanylate-binding protein (GBP) family, particularly mouse GBPs, where mouse models can be used to assess their antiviral properties in vivo. Herein, we demonstrate that mouse GBP1 (mGBP1) was upregulated in a mouse airway epithelial cell line (LA-4 cells) following pretreatment with mouse IFNα or infection by influenza A virus (IAV..
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Awarded by University of Melbourne
Funding Acknowledgements
We acknowledge the Melbourne Cytometry Platform (Peter Doherty Institute node) for provision of flow cytometry services. Fluorescent images were taken by confocal microscopes located at the Biological Optical Microscopy Platform, The University of Melbourne. We also thank Marco Herold at the Walter and Eliza Hall Institute for provision of the lentiviral constructs. This study was supported by Project Grant Number APP1143154 from The National Health and Medical Research Council (NHMRC) of Australia. The Melbourne WHO Collaborating Centre for Reference and Research on Influenza is supported by the Australian Government Department of Health and Aging. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.